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Fig. 2. LIMK is active in developing RGCs and their processes. (A) The P-LIMK antibody detects the phosphorylated, active forms of LIMK1/2 with the molecular weight of 65 kDa. (B–D) Transverse cryosections were immunolabelled with the antibody, and stacked optical slices presented. At stage 33/34, there is label in RGC axons, shown in a section (B) where RGC axons run along the vitreal surface of the retina (arrows) and in the optic nerve head, and in a section (C) that has captured part of the bilateral optic nerves before they enter the ventral diencephalon (white arrowheads). Labelling of the brain neuropil is also evident. At stage 37/38 (D), the optic fiber layer expression is still present (white arrows), but there is now more obvious labelling of the IPL. cmz, ciliary marginal zone; Di, diencephalon; D, dorsal; IPL, inner plexiform layer; L, lens; onh, optic nerve head; PE, pigment epithelium; RGCL, retinal ganglion cell layer; V, ventral. |
