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Fig. 3. Sections of embryos analyzed by whole-mount in situ hybridization for XmafB expression. (A–G) Sectioning planes are indicated in schematic diagrams. (A) At stage 14 XmafB is expressed in the anterior neuroectoderm. (B) At stage 22, signal also appears in neural crest cells (arrow). (C) Stage 24. Staining is observed in the dorsal part of the neural tube. (D) Section of stage-23 embryo at the level of the trunk showing XmafB expression in somite (arrowhead) and blood island (white arrow). (E–H) Stage 32. (E) Section at the level of rhombomere (r) 4. Bilateral staining is seen in the dorsal part of the neural tube (arrow). (F) Section through r5 and pronephros. Expression of XmafB is found in the dorsal part of the neural tube (arrowhead) and developing pronephros (arrow). (G) Section at the level of the trunk showing XmafB expression in somite (arrowhead). (H) Parasagittal section shows myotomal cells are stained. Scale bars: 200 μm. |
