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Fig. 5. Transverse sections through the level of the eye showing expression of XmafB (A–G) and XL-maf (A′–G′) during lens formation, after whole-mount in situ hybridization. (A,A′) At stage 23 expression of XmafB is found in the sensorial layer of the ectoderm, but not XL-maf. (B,B′) Both genes are expressed in the sensorial layer which is beginning to thicken at stage 27. (C,C′) Stage 28. (D,D′) At stage 31 signals are detected in the developing lens placode. (E,E′) At stage 32 XmafB is detectable in the border between the ectoderm and lens placode, while XL-maf can be detected in the prospective lens fiber cells of the placode. (F,F′) Stage 33/34. XL-maf is also expressed in the prospective neural retina (arrows). (G,G′) Subsequently expression of XmafB is found in lens epithelium, and that of XL-maf in lens fiber cells at stage 37/38. he, head ectoderm; l, lens; le, lens epithelium; lf, lens fiber cells; lp, lens placode; oc, optic cup; ov, optic vesicle. Scale bars: 100 μm. |
