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Fig. 3 Distribution of X-ptf-a and X-ptf-B transcripts in tailbud stage embryos. A digoxigenin-labeled cRNA probe was prepared by in vitro synthesis using SP6 RNA polymerase from the X-ptf-a or X-ptf-B coding regions on pSP64-poly(A). Whole mount in situ hybridization transcript distribution from the side. (B) and (C) X-ptf-B transcript distribution from the side and top, respectively. |
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mdk.L (midkine) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 28, lateral view, anterior left, dorsal up. |
