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Figure 1. Broad expression of malectin in embryonic
and adult X. laevis. (A) Analysis of embryos by whole
mount in situ hybridization showing malectin expression
in the anterior neuroectoderm (ne) and neural crest
(nc) at stages 18 and 20 (A1, A2), and at later stages, e.g.,
32, in the hatching gland (hg), retina (re), otic vesicle
(ot), epibranchial placodes (eb), pronephros (pn), and
the tail tip (tp); anterior (a); posterior (p). At stage 41
(A4), transcripts are detected in the liver (li), dorsal and
ventral pancreas (dp and vp), branchial arches (ba), and
the proctodeum (pd). (B) Analysis of embryonic expression
of malectin by RT-PCR showing expression in the
oocyte (stage VI) and continued expression throughout
development, and the contrasting expression of protein
disulfide isomerase, xPDIp, only from late tadpole stage
39 onward. Abbreviations: stage VI oocyte (VI); unfertilized
egg (0) and fertilized egg (1). (C) The expression analysis in adult tissue by semiquantitative RT-PCR showing a broad distribution
of malectin in comparison with xPDIp, which is detected in pancreas and stomach only. Additional abbreviations not defined in A: gall
bladder (gb); heart (he); intestine (in); kidney (ki); lung (lu); muscle (mu); pancreas (pa); stomach (st). |
