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Figure 6. Proliferating cells are musashi1-expressing neural progenitor cells. Stage 47 tadpoles were injured in the right tectum. Two days later animals were exposed to CldU for 2 hours and processed immediately for CldU immunolabeling. A–F: Representative single optical sections from confocal images of 30-μm sections through the optic tectum of tadpoles labeled with antibodies to musashi1 (Msi1, green) and CldU (magenta). D–F: enlargements of the boxed area marked in C. 93.3 ± 2.5% of CldU-positive cells in injured tectal lobes were double-labeled with musashi1 antibodies (n = 7 animals). Scale bars = 50 μm in C and 20 μm in F. |
