| |
Fig. 2. Localization of c-myc RNA during oogenesis. In situ hybridization of myc 35S-DNA probes (A,B) and myc
antisense 35S-RNA probes (C-F) to Xenopus oocytes sections. A high level of labelling was detected throughout the
cytoplasm of young oocytes both with DNA (A,B) or RNA probes (C,D). In later stage-Ill and -IV oocytes (E,F), the
density of silver grains is lower and still uniformly distributed in the cytoplasm. The white ring around the oocyte
(arrow) is due to pigment granules in the cortex of wild-type oocyte. (G) Hybridization with a pUC18 DNA probe and
(H) with a sense myc RNA probe: no signal above background can be observed. Labels as Fig. 1. (A,C,E-H) Darkfield
illumination; (B,D) brightfield illumination. (E) X120; (A,F,G) X130; (B,C,D,H) X180. |
