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Fig. S3. Endogenous Miz-1 transcripts are maternally provided (A) Scheme of human Miz-1 showing the position of the Zn-finger region, the amplification product of the RT-PCR shown in (B) and the fragment of xMiz-1 used for in situ hybridization in (C). (B) RT-PCR reveals that Xenopus Miz-1 is maternally expressed. Expression persists until neurula stage. Expression of histone H4 was used as control. Bottom panel shows control RT-PCR without reverse transcriptase. (C) In situ hybridizations of gastrula stages for xMiz-1 and xMyc. Embryos were transversally cut into halves to show the expression pattern from inside. |
