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Fig. 2. Whole mount in situ hybridization of X. laevis embryos with the full-length XMRF4 probe or XMyf5 probe. All are oriented anterior to the right. (A) Stage 14- 15 early neurula, dorsal view. XMRF4 expression is seen in
presomitic mesoderm prior to segmentation, as well as in the anterior. (B) Stage 20 neurula, lateral view. XMRF4 staining is most intense in the somites but also evident in the eye primordia. (C) Stage 16 neurula, dorsolateral view. XMyf5 expression is confined to the posterior mesoderm.
(D) Stage 31-32 tailbud embryo, lateral view. In addition to the myotomal staining, XMRF4 expression is evident in the eyes, brain, branchial arches, otic vesicles, and head meso- derm. (E) Stage 31-32 tailbud embryo, lateral view. XMyf5 expression is seen in tailbud mesoderm, dorsal and ventral myotomal cells, and in primordia of some cranial muscles. |
