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Figure 5. Spatial expression patterns of Xtcat-2 mRNA analyzed by whole-mount in situ hybridization. A: Fertilized egg (St.1). B: Eight-cell stage embryo (St. 4). C: Mid-gastrula (St. 11). D: Late neurula (St.18). E: Early tail bud (St. 26). F: Late tail bud (St. 30). G: No cells expressing Xtcat-2 mRNA are observed at St. 40. H,I: Section of Xtcat-2 stained fertilized (H) or eight-cell stage (I) embryo revealed the localized expression of Xcat-2 mRNA. J,K: Sagittal section of Xtcat-2-stained St. 11 (J) or St. 18 (K) embryo. L,M: Transverse section of tail bud stained with Xtcat-2 at the same stage of E and F, respectively. Scale bars = 500 mu m in A-G, 200 mu m in H-M. |
