|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
nkx2-1
|
|
laevis
|
NF stage 29 and 30
|
forebrain
,
ventral
|
neurog2
|
|
tropicalis
|
NF stage 29 and 30
|
brain
,
midbrain
,
hindbrain
,
spinal cord
,
ventricular zone
,
[+]
|
neurog2
|
|
laevis
|
NF stage 29 and 30
|
olfactory placode
,
forebrain
,
midbrain
,
hindbrain
,
spinal cord
,
[+]
|
tubb2b
|
|
tropicalis
|
NF stage 29 and 30
|
midbrain
,
trigeminal placode
|
tubb2b
|
|
laevis
|
NF stage 29 and 30
|
otic vesicle
,
retina
,
midbrain
,
trigeminal placode
|
neurog1
|
|
laevis
|
NF stage 29 and 30
|
otic vesicle
,
midbrain
,
hindbrain
,
pineal gland
,
trigeminal placode
,
[+]
|
en2
|
|
laevis
|
NF stage 29 and 30
|
midbrain-hindbrain boundary
|
neurog3
|
|
tropicalis
|
NF stage 29 and 30
|
hindbrain
,
spinal cord
|
neurog3
|
|
laevis
|
NF stage 29 and 30
|
hindbrain
,
spinal cord
|
|
|
Figure 4. A: Comparative in situ hybridization analysis of X. tropicalis Ngn1, Ngn2, Ngn3, and X. laevis Ngn1, Ngnr-1, and Ngn3 in stage 30 embryos. Probes are indicated on the left of each line of embryos. Shown: first panel, lateral view, anterior to the left; second panel, close-up of the head region; third panel dorsal view of head region; fourth panel, transverse section at the level of the retina; fifth panel, transverse section at the level of the otic vesicle. The transversal sections were performed with embryos stained for both Ngn (dark blue) and N-tubulin (red). VII, facial epibranchial placode; IX, glossopharyngeal epibranchial placode; X1, first vagal epibranchial placode; X2, second vagal epibranchial placode; X3, third vagal epibranchial placode; hb, hindbrain; mb, midbrain; nc, notochord; op, olfactory placode; vOT, otic vesicle; tg, trigeminal placode; pg, pineal gland; pP, posterior lateral line placode; pM, middle lateral line placode; pAD, anterodorsal lateral line placode; r, retina. B: Double in situ hybridization of stage 30 X. laevis embryos with Ngn1, Ngnr-1, Ngn3 (dark blue) and the mid-hindbrain boundary marker En-2 (red) or with the ventral forebrain marker Nkx2.1 (red) (transversal section). Black arrowhead marks the expression of Ngn3 in the ventral forebrain. |