| |
Fig. 7. Paraffin sections of control and mutant embryos following
whole-mount in situ analyses for XNkx2-5 and XcTnI mRNAs.
Sections shown were from stage 28 embryos, co-stained for XNkx2-5
mRNA (brownish-purple) and for XcTnI mRNA (blue); dorsal at the
top. (A) Control embryo exhibits symmetrical staining of both XcTnI
mRNA, in differentiating myocardial cells, and XNkx2-5 mRNA in
both differentiating myocardial cells and adjacent mesoderm.
(B) Mutant embryo exhibits asymmetrical staining of both XcTnI and
XNkx2-5 mRNAs, with one side (arrow) exhibiting greatly reduced
signal for both mRNAs. M, midgut; N, notochord; O, otic vesicle and
R, rhombencephalon. |
