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Fig. 5. xPN-1 inhibits expression of a subset of mesendoderm genes in whole embryos. Embryos were injected with 2 ng of each RNA into two dorsal blastomeres at the four-cell stage, and fixed at Stage 10.5 for in situ hybridization analysis. Embryo is in vegetal view and the dorsal side is up. (A–D) in situ hybridization of Xbra. Embryos were injected with EGFP (A), xPN-1 (B), xPN-1 pm (C) or xPN-1 δC RNA (D). xPN-1 and xPN-1 δC inhibited Xbra expression on the dorsal side (red arrows). xPN-1 pm caused weak Xbra expression (red arrows). (E–H) in situ hybridization of chordin (chd). Embryos were injected with EGFP (E), xPN-1 (F), xPN-1 pm (G) or xPN-1 δC RNA (H). chordin expression was not inhibited in each condition. (I–L) in situ hybridization of cerberus (cer). Embryos were injected with EGFP (I), xPN-1 (J), xPN-1 pm (K) or xPN-1 δC RNA (L). xPN-1 and xPN-1 δC inhibited cerberus expression on the dorsal side (red arrows). (M–P) in situ hybridization of goosecoid (gsc). Embryos were injected with EGFP (M), xPN-1 (N), xPN-1 pm (O) or xPN-1 δC RNA (P). goosecoid expression was not inhibited in each condition. (Q–T) in situ hybridization of Xnr1. Embryos were injected with EGFP (Q), xPN-1 (R), xPN-1 pm (S) or xPN-1 δC RNA (T). Xnr1 expression was not inhibited in any condition. |
