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notch2xenopus anterior 

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Experiment details for notch2

Murato Y and Hashimoto C (2009) Assay

Xhairy2 functions in Xenopus lens development by regulating p27(xic1) expression.

Gene Clone Species Stages Anatomy
notch2.S laevis NF stage 15 anterior

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  Figure 4. Xhairy2 knockdown reduces the expression of LF marker genes in neurulae without marked changes in PPE marker expression. Micro-injection and lineage tracing were performed as described in Figure 3. Injected MO is indicated at the upper right corner and WISH probe is indicated at the lower right corner of each panel. a: Schematic presentation of LF in neurulae. b-i: LF marker genes. b, c: Two-color WISH of st.-15 embryos to confirm LF position. LF expression (black arrowheads) of pax6 (magenta) and six3 (purple) is indeed outside of the neural plate border that is marked by Xhairy2 (light blue). j, k: Embryos were injected with 6.9 ng of Xhairy2 MO and/or 20 pg of Xhairy2b mRNA where Xhairy2 MO does not bind in order to check the specificity of Xhairy2 MO to LF-loss phenotypes. I: Quantitative summary of j and k. Error bar: standard deviation of 5 independent experiments. m-t: PPE marker genes. Red arrowheads indicate reduction of marker gene expression. All the samples were pictured from the anterior side with the dorsal side up.

Gene Clone Species Stages Anatomy
notch2.S laevis NF stage 28 anterior

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  Figure 3. Effects of Xhairy2 knockdown on the expression of lens and retinal marker genes in tail-bud-stage embryos. Co MO (3.4 ng: a-f') or Xhairy2 MO (6.9 ng: g-l'), together with EYFP and lacZ mRNA, was micro-injected into one dorsal animal blastomere at the 8-cell stage, and the embryos were harvested at st. 25 (for six6), st. 28 (for notch2, foxE3, L-maf, gamma 1-cry), or st. 32 (for rx1), followed by WISH. MO-receiving cells were visualized by X-gal staining (green). Red arrowheads indicate reduction of marker gene expression. Note that Xhairy2 knockdown reduced the expression of LP marker genes, while virtually no effect was seen on the expression of retinal marker genes six6 and rx1.