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Fig. 6. Whole-mount in situ hybridization of the Xenopus NP1 gene in the regenerating tadpole tails. A,B: Whole-mount in situ hybridization was performed using Xenopus tadpole regenerating tails 7 days after amputation and antisense (A) or sense (B) digoxigenin- labeled RNA probes. The boundary between the normal tail portions and the regenerating tail is indicated by white arrowheads, and the boundary between the regenerating tail and the most distal portions is indicated by black arrowheads. C: The tadpole regenerating tail specimen stained by whole-mount in situ hybridization was sectioned to observe the inner tissues. The boundary between the most distal tip without NP1 expression (right) and the NP1-expressing regenerating tail (left) is indicated by filled red arrowheads. The portion corresponding to the distal tip is outlined by open red arrowheads. |
