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Fig. 7. CPG15 is targeted to retinal ganglion cell axons. A: CPG15
immunoreactivity in stage 42 is detected in the optic nerve (ON) and
optic chiasm (OX). B: Retinal axons within the optic nerve in stage 47.
C: High-magnification image of the optic nerve head of retina from
stage 47. Note the CPG15 IR around cells in the RGC layer and the
concentrated immunoreactivity in the optic nerve. D: Preimmune
serum does not label RGC somata or axons. E: In retinal explants,
CPG15 IR can be observed all along the length of axons, including the
growth cone. F: Double staining with rhodamine-phalloidin highlights
actin-based axonal structure. Scale bar 5 100 mm for A,B,D; 50 mm
for C. |
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Fig. 8. CPG15 expression in tadpole brain. A–E: Horizontal cryostat
sections from a stage 45 tadpole shown in sequence from dorsal
through ventral. CPG15 immunohistochemistry shows cell bodies labeled
in a honeycomb pattern throughout the brain. Cells are labeled
in the olfactory bulb (OB), optic tectum (OT), telencephalon (TL), and
tegmentum (Te). Intense labeling occurs in axon tracts, notably the
optic chiasm (OX), retinotectal axons (RT) in the optic tectal neuropil,
tectotegmental axons (TT), and tectospinal (TS) axons coursing toward
hindbrain (HB) and spinal cord. Scale bar 5 100 mm. |
