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Fig. 6. Distinct effects of expression of N and DN-C in Xenopus embryos.
Uninjected embryos (WT), and embryos injected with N, or DN-C were
photographed at tadpole stage (A) or analyzed by in situ hybridization (B,
C). (A) Cement glands (arrow) and other anterior structures are enlarged in
N-injected embryos. In contrast, embryos injected with DN-C display a
smaller cement gland, reduced anterior structures, and enhanced posterior
structures. (B) In situ hybridization was performed with a pan neural RNP
marker. Arrows indicate the otic vesicle. The neural tissue anterior to the
otic vesicle is enlarged in N-injected embryos, and reduced in DN-Cinjected
embryos as compared to control embryos. (C) In situ hybridization
was performed with the forebrain marker Otx-A. The pattern of Otx-A
expression (arrows) is expanded in N-injected embryos while expression in
DN-C-injected embryos is similar, but reduced in comparison to that of
control embryos. |
