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Fig. 4. spry3 expression is dependent on BDNF-TrkB. (A) Synexpression group of spry3. The pattern of expression of spry3 resembles that of bdnf and trkb. (B) Total RNA from whole embryos or isolated neural tube (stage 26) was purified from Xenopus embryos injected with morpholinos against bdnf (MO BDNF) or trkb (MO TrkB) or with a control morpholino (MOC). spry3 expression was assessed by real-time PCR using rpl8 as a reference. Knocking down bdnf and trkb results in a significant decrease in spry3 expression (*P<0.005, using a statistical ANOVA test). Error bars indicate mean ± s.e.m. (C) Xenopus embryos were injected at the 1-cell stage with MO BDNF, MO TrkB or MOC, fixed at stage 28 and processed for whole-mount in situ hybridisation using a probe specific for spry3. Embryos are scored as normal/weak when spry3 expression is detected both in the trigeminal nerve and the spinal cord, as medium when spry3 expression is only partially detected in the spinal cord and as strong when no expression of spry3 is detected. The percentage of affected embryos is indicated in each case. |
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ntrk2 (neurotrophic tyrosine kinase, receptor, type 2) gene expression in Xenopus tropicalis embryo as assayed by in situ hybridization, NF stage 28, lateral view, dorsal up, anterior left. |
