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Fig. 4. Cellular localization of XOMP gene expression in adult
Xenopus olfactory epithelium by in situ hybridization. A: Section
through the medial diverticulum (MD) incubated with a digoxigeninlabeled
antisense riboprobe of XOMP2. Labeled cells are confined to
the sensory part of the mucosa; no signals were detected in the
nonsensory part. Arrowheads indicate the transition between the
olfactory epithelium (OE) and adjacent respiratory epithelium (RE).
B: A section through the lateral diverticulum (LD) probed with
XOMP1. Within the epithelium, specific hybridization is restricted to
olfactory receptor neurons (ORN) but not to sustentacular cells (SC) or
basal cells (BC). Signals are absent from the lamina propria (LP).
Scale bars 5 100 μm in A, 40 μm in B. |
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Fig. 5. In situ hybridization of adult Xenopus coronal sections
through the sensory epithelia of all subcompartments probed with
either XOMP1-specific or XOMP2-specific antisense RNA. A,B: Adjacent
sections through the lateral diverticulum (LD) hybridized with
XOMP1 and XOMP2. XOMP1 is strongly expressed in the sensory
cells of the LD (A). The expression of XOMP2 is significantly weaker
(B). C,D: Consecutive sections through the medial diverticulum (MD)
probed with XOMP1 and XOMP2. C: XOMP1 strongly hybridized to
only single neurons randomly distributed within the MD. The majority
of neurons are weakly stained. D: Hybridization with the XOMP2-
specific probe intensively labeled many cells in the olfactory neuron
layer. E: Coronal section of the vomeronasal organ (VNO) annealed
with XOMP1. The probe labeled single, randomly distributed chemosensory
cells in the VNO. These XOMP1-expressing neurons are
surrounded by cells with a diffuse and very weak staining. Arrowhead
indicates a single mature VNO neuron expressing XOMP1. Scale
bars 5 50 μm. |
