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Fig. 4. Nbx acts as a transcriptional suppressor. (A) Schematic representation
of Nbx, VP-Nbx, and EnR-Nbx constructs. (B) Phenotypes of Nbx
(200 pg)-injected embryos. The Nbx injection into the dorsal side caused a
head defect. (C) Uninjected embryos developed normally. (D, E) Wholemount
in situ hybridization analysis of Nbx (200 pg)- and -gal (100
pg)-injected embryos at stage 11. Nbx suppressed the organizer markers
Otx2 (D) and goosecoid (E). (F, G) Whole-mount in situ hybridization
analysis of -gal (100 pg)-injected embryos. -gal injection did not affect
the expression of Otx2 (F), goosecoid (G). (H) Phenotype of VP-Nbx (100
pg)-injected embryo. The VP-Nbx injection into the ventral side caused
ectopic cement gland formation (arrowhead in H). (I:) Uninjected embryos
developed normally. (J�M) Whole-mount in situ hybridization analysis of
VP-Nbx (100 pg)- and -gal (100 pg)-injected embryos. VP-Nbx injection
into the dorsal side led to the expanded expression of Otx2 (J) and
goosecoid (K). VP-Nbx injection into the ventral side resulted in the
ectopic expression of Otx2 (L) and goosecoid (M) at the injected region.
(N) Phenotype of EnR-Nbx (100 pg)-injected embryo. The injection caused
a head defect. (O:) Uninjected embryos developed normally. |
