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FIGURE 8.
Knockdown of Dhrs3 interferes with somitogenesis. Dhrs3 MO (30 ng) was injected at the two-cell stage, and the morphants were collected at stage 28 for immunostaining and whole mount in situ hybridization analysis. A-B′, immunostaining with 12/101 antibody indicated that in Dhrs3 morphants the somites showed a diffused pattern and a reduction in immunoreactivity. C-H′, expression of somite marker genes myoD, pax3, and fgf8 on the dorsal side of the embryos showed the segmented expression pattern of somite units in the control embryos (C′-E′, arrow) was not observed in Dhrs3 morphants (F′-H′). I, real time PCR indicated that expression level of myoD, pax3, and fgf8 at stage 21 was down-regulated in Dhrs3 morphants. |
