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Fig. 7. Positioning of Pax gene expression domains relative to Sox3 as revealed by whole mount double in situ hybridization. Dorsal views (A, D, and G) of neural plate stage Xenopus embryos (stage 14; anterior to the left) and lateral (B, E, and H) and oblique dorsolateral (C, F, and I) views of neural fold stage Xenopus embryos (stage 16; anterior to the left) demonstrating the position of Pax6 (A–C), Pax3 (D–F), and Pax8 (G–I) expression domains relative to Sox3 expression. Inserts in A, D, and G show Sox3 expression individually for clarification. Asterisks indicate lateral domain of Sox3 expression. Arrows indicate anterior and posterior borders of placodal expression domains for Pax6 (green arrows: prospective lens and trigeminal placodes, A–C), Pax3 (yellow arrows: prospective profundal placode, D–F), and Pax8 (brown arrows: posterior placodal area, G–I). The green arrowhead in B and C indicates Pax6 expression in the lateral part of the anterior placodal area (prospective olfactory placode). Inserts in B, E, and H identify the corresponding subdomains of Six1 expression. |
