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Figure 3. Promoter-driven GFP mRNA expression recapitulates endogenous XPAPC expression in spatial and temporal-specific manner. Transcripts of the endogenous XPAPC or the GFP reporter gene were detected by whole mount in situ hybridization with XPAPC or GFP antisense probes, respectively. A-E: Expression of endogenous XPAPC RNA at the stages indicated. F-J: Expression of the pFLGFP in transgenic embryos at the same stages. The expression pattern of GFP mRNA was reminiscent of the endogenous XPAPC expression pattern. A, B and F-J: Vegetal views of gastrula with the dorsal side up; (C and H) dorsal views of neurulae with anterior side up; (D and I) lateral views of neurulae, anterior to the left; (E and J) lateral views of tadpoles, anterior to the left. |
