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FIG. 5. Localization of PCNA protein in oocytes and embryos. Immunolocalization with wPCNA antibody was with oocyte and embryo cut
sections as described under Materials and Methods. (A) Overview of a cut section of oocytes from stages II to VI of oogenesis (Dumont, 1972). (B)
Immunoblotting analysis with the wGSIL antibody of cytoplasmic (lane 1) and germinal vesicle (lane 2) fractions of an oocyte stage VI. (C) and
(D) are enlargements of oocytes stage VI after incubation with a-PCNA and rabbit nonimmune serum, respectively. (E) Cut section of a
gastrula. Arrows indicate the nuclei. |
