| |
Fig. 1.
Dystrophin is undetectable in the contact zone between the myofiber and dividing satellite cells. (A) Xenopus body musculature stage 54. PCNA-positive cell (red) over a region without dystrophin (cyan). Insert: detail of the dystrophin gap in the area of contact (small arrow in main image). Scale bar: 25 µm. (B) Xenopus tail stage 53 (3 days after tail amputation). pHH3 (red)-positive cell in close contact with the myofiber over a region without dystrophin (cyan). Insert: detail of the dystrophin gap in the contact area (small arrow in main image). Scale bar: 25 µm. (C) Mouse muscle 7 days after birth. Pax7 (green)-PCNA (red)-positive cell over undetectable dystrophin (cyan). Insert: detail of the dystrophin gap in the contact area (small arrow in main image). Scale bar: 10 µm. (D) Mouse muscle 30 days after birth. Pax7 (green)- PCNA (red)-positive cell over undetectable dystrophin (cyan). Insert: detail of the dystrophin gap in the contact area (small arrow in main image). Scale bar: 10 µm. (E) 3-month-old mouse muscle, 6 days after cardiotoxin-induced injury. Pax7 (green)- PCNA (red)-positive cell over undetectable dystrophin (cyan). Insert: detail of the dystrophin gap in the contact area (small arrow in main image). Scale bar: 25 µm. (F) Xenopus limb bud, stage 57. Area without a plasma membrane between the satellite cell chromosomes and the sarcomeres (arrow). Insert: lower magnification showing the chromosomes of the satellite cell and the area observed (small arrow). Scale bar: 0.2 µm. (G) Xenopus tail stage 53; 14 days after amputation. Contact area between one satellite cell and the myofiber, without a plasma membrane between them (arrow). Insert: lower magnification showing the chromosomes of the satellite cell and the area observed (small arrow). Scale bar: 0.2 µm. (H) Mouse muscle 7 days after birth. Area without a plasma membrane between the satellite cell cytoplasm and myofiber cytoplasm. Part of the myofiber cytoplasm, with a clear plasma membrane, surrounds the satellite cell (red arrow). Insert: lower magnification showing the chromosomes of the satellite cell and the area observed (small arrow). Scale bar: 0.5 µm. |
