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Figure 4. Spatial distribution of PC6 transcripts during Xenopus embryogenesis. A-H: In situ hybridization of probes specific for all xPC6 isoforms (A-F) or for xPC6B-D (G,H) to whole Xenopus embryos. Dorsal (A) and dorsoanterior (B) views of neurula (st. 18) stage embryos or lateral views of tail bud (st. 28-30; C-E,G) and tadpole (st. 36; F,H) stage embryos are shown. D: The embryo was cleared to visualize internal structures. nf, neural fold; e, eye; pd, pronephric duct; ov, otic vesicle; pa, pharyngeal arches; nc, notochord; n, nasal placode; ll, lateral line; gs, gill slits; k, kidney; PC, proprotein convertases. Arrowheads denote staining of specific stripes of cells in the brain, open arrowheads indicate staining of mesenchymal cells posterior to the pharyngeal arches. |
