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Fig. 1. Expression patterns of XPteg (A) RT-PCR showing temporal expression pattern of XPteg. Numbers above the lanes indicate the embryonic stages. ODC serves as a loading control. –RT, control in the absence of reverse transcriptase. W.E., whole embryo. (B) Spatial expression pattern of XPteg analyzed by in situ hybridization. (a) Dorsal view of expression of XPteg in dorsolateral mesoderm (dlm) in late gastrulae. A, anterior; P, posterior. (b and c). Dorso-anterior and lateral views of XPteg expressed in the pronephric anlage (pa) at stage 14 and 16, respectively. (d) XPteg was observed in the intermediate mesoderm at stage 21 (dorsal view). (e) Transverse section of the embryo shown in panel (d). (f) Ventro-anterior view showing XPteg expressed in the eye placode (ep) and blood islands (bi) at stage 23. (g) Transverse section of the embryo in panel (f), which shows XPteg-positive blood islands. (h–j) Pronephros (pn)-specific expression of XPteg at the tadpole stages.(k–m). Enlarged views of XPteg localized to proximal tubules (pt) at stage 32, 36, and 38, respectively. (n) Transverse section of the tubules shown in panel (m). (o and p). Transverse and sagittal sections in situ hybridization of a stage 40 embryo showing XPteg-expressing proximal tubules. |
