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Fig. 2. Pfk1 spatiotemporal expression pattern in Xenopus laevis embryos. Pfk1 expression was analysed by WISH in embryos from the blastula stage 9 until the tailbud stage 22. Expression was observed at cleavage and gastrulation stages (see sense probe staining in Supplemental Fig. 2). Increased expression was observed during later development, notably in the dorsal tissues. (A) Stage 9 blastula embryo shown in lateral view, animal pole on the right; (B) Embryos at gastrula stage 10, 5 and 12 shown in
lateral view, dorsal on top (white arrows indicate the blastopore lip); (D) Embryos at neurula stages 14 and 18 shown in dorsal view, anterior on the bottom (red arrows indicate the neural plate and yellow arrows the neural crest and the preplacodal field). (F) Stage 22 tailbud embryo shown in lateral view, anterior on the right (red arrow indicates the dorsal side of the embryo; yellow arrows indicate the prospective craniofacial region). Bar = 1 mm. |
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Supplementary Fig. 2.
Pfk1 expression pattern in Xenopus laevis embryos. Pfk1 expression is shown in X. laevis embryos from blastula stage 9 until tailbud stage 22. A sense probe control (A-C, G-I) and an antisense probe staining (D-F, J-L) are shown at each stage of development. (A-F) Embryos are shown in lateral view, animal pole on the right (A, D) or dorsal on top (B, C, E, F). (G, H, J, K) Embryos are shown in dorsal view, anterior on the bottom. (I, L) Embryos are shown in lateral view, anterior on the right. White arrows (B, C, E, F) indicate the blastopore lip. Red arrows (J, K) indicate the neural plate and (L) the dorsal side of the embryo. Yellow arrows (J, K) indicate the neural crest and the preplacodal field and (L) the prospective craniofacial region. Bar = 1 mm |
