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FIG. 1. XPcl1 sequence comparison and expression pattern. XPcl1 is compared (A) to mouse Pcl1 (TLH and SMH, unpublished results),
M96A (Inouye et al., 1994), and Drosophila Pcl (Lonie et al., 1994). Percentages are amino acid sequence identity of the indicated region
relative to XPcl1. The conserved Cys4-His-Cys3 PHD1 and PHD2 domains (Aasland et al., 1995), homology regions II and III, and the region
of microheterogeneity in XPcl1 (*) are indicated. GenBank accession numbers are XPcl1 (AF130453) and mPcl1 (U81490). XPcl1 mRNA
levels were quantified during Xenopus embryogenesis using reverse transcription followed by PCR with gene-specific primers (B). Primers
specific for Xenopus ornithine decarboxylase (ODC) were used as control to correct for differences in RNA levels and reverse transcription
efficiency. Developmental stage is indicated (Nieuwkoop and Faber, 1967). Cycle number and product sizes are XPcl1 (27 cycles, 436 bp)
and ODC (32 cycles, 234 bp). XPcl1 antisense probe was used for whole-mount RNA in situ hybridization (C–H). XPcl1 expression is
restricted to the brain (white arrowhead), eyes (white arrow), and otic vesicles (black arrow) as shown at Stage 33, lateral view (C, E), and
in a dorsal view (D) at Stage 26. Inset of (C) shows an embryo hybridized with sense XPcl1 probe. The embryo in (E) has been cleared and
shows apparent gaps of expression in the brain. Transverse sections of stage 37 embryos after XPcl1 detection are also shown (F, G, H).
Signal is present in the brain, eyes (e), and otic vesicles (o). |
