| |
Fig. 3. xPKD1 expression in adult tissues. A. RT-PCR experiments were
conducted using xPKD1 specific primers (see Materials and methods; the
amplicon size is approximately 400 bp) and RNA extracted from ovaries
(lane 1), heart (lane 2), kidney (lane 3), liver (lane 4), intestine (lane 5) and
lung (lane 6). B. Control RT-PCR experiments were conducted using ODCspecific
primers and the same RNAs samples as in A. C. Northern blot
analysis of the xPKD1 transcript in RNAs extracted from ovaries (1) or
adult kidney (2). The blots were then probed with the 714 bp external
PKD1 RT-PCR product. The exposure time was 48 h. The 14 kb xPKD1
transcript is indicated. |
