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Fig. 5. Expression of fatvg mRNA in the fat bodies. (A) fatvg was detected in the fat bodies in stage 56 tadpoles by in situ hybridization. The vacuole-like structures were fat droplets. Hybridization signals were detected in the cytoplasm, which had been displaced to the peripheries of the cells by the fat droplets. (B) Hoechst staining of the section in (A) to show the positions of the nuclei on the peripheries of the cells in the fat bodies. (C) No staining was detected when the sense probe was used. (D) Hoechst staining of the section as shown in (C). |