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pmp22xenopus profundal placode [+] 

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Experiment details for pmp22

Tae HJ et al. (2015) Assay

Temporal and spatial expression analysis of peripheral myelin protein 22 (Pmp22) in developing Xenopus.

Gene Clone Species Stages Anatomy
pmp22.L laevis NF stage 22 profundal placode

  pmp22 (peripheral myelin protein 22) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 22, lateral view, anterior left, dorsal up.

Gene Clone Species Stages Anatomy
pmp22.L laevis NF stage 26 profundal placode
pmp22.L laevis NF stage 28 profundus ganglion

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  Fig. 2. Expression of Pmp22 in whole-mount in situ hybridization. (A) Expression of Pmp22 at stage 15 in the cement gland. Anterior view, dorsal to top. (B) At stage 22, Pmp22 was detected in the developing somites and profundal and trigeminal nerves. (C) At stage 22 Pmp22 sense probe was used as a negative control. (D) Pmp22 was expressed in the profundal and trigeminal ganglia, cranial mesenchyme, and somites at stage 29/30, where it was also beginning to be expressed in the branchial arches. (E) Strong expression of Pmp22 was observed in the craniofacial region. (F–G, I) To confirm the tissue-specific expression of Pmp22, we also performed in situ hybridization for Sox9 and Islet1. (H, I) At stage 35, both Pmp22 and Sox9 were detected in the branchial arches. Panels (B–I), lateral view, anterior to the left. ba, branchial arches; cg, cement gland; pr, profundal ganglia; s, somites; tg, trigeminal ganglia.

Gene Clone Species Stages Anatomy
pmp22.L laevis NF stage 29 and 30 profundus ganglion

  pmp22 (peripheral myelin protein 22) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 30, lateral view, anterior left, dorsal up.