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Fig. 2. Expression patterns promoted by different Pou3f4 HCNRs in Xenopus and zebrafish transgenic assays. Xenopus embryos show in situ hybridization to detect EGFP mRNA in fixed transgenic animals while zebrafish embryos show EGFP in living transgenic specimens. All panels, except (l) that show a dorsal view, are lateral views of transgenic Xenopus (a, c, e, g, i) at stage 35 or transgenic zebrafish (b, d, f, h, j, l) at 36–72-h post fertilization (hpf). The position of the HCNRs in the human genome is shown in the upper right corner of each panel. Different tissues are pointed at with coloured arrowheads. Blue otic vesicle, red midbrain, orange hindbrain, yellow kidney and purple pancreas. Note the equivalent patterns promoted by each HCNR in Xenopus and zebrafish transgenic embryos. k Expression pattern of XenopusPou3f4 |