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FIG. 7. Double staining for BrdU uptake and gene expression in stage 40 Xenopus CMZ. (A) Double staining for BrdU uptake and Xath5 expression. Xath5 staining is shown in A, BrdU immunostaining in B. Double staining in C shows that BrdUﰃ cells in the peripheral CMZ are Xath5ﰄ. In the central CMZ, BrdUﰃ cells are stained for Xath5. A few cells are BrdUﰄ and stained with Xath5. (D) Double staining for BrdU uptake and neuroD expression. neuroD staining is shown in D, BrdU immunostaining in E. Double staining in F shows that BrdUﰃ cells in the peripheral CMZ are neuroDﰄ. In the central CMZ, BrdUﰃ cells are stained for neuroD. neuroDﰃ cells in the central retina are BrdUﰄ. (G) Double staining for BrdU uptake and Brn-3.0 expression. Brn-3.0 staining is shown in G, BrdU immunostaining in H. Double staining in I shows that all BrdUﰃ cells in the CMZ are Brn-3.0ﰄ and that all Brn-3.0ﰃ cells are BrdUﰄ. (J) Double staining for BrdU uptake and Pax6 expression. Pax6 staining is shown in J, BrdU immunostaining in K. Double staining in L shows that BrdUﰃ cells in the CMZ express a low level of Pax6 and that cells in the central retina expressing a strong level of Pax6 are BrdUﰄ. Peripheral CMZ is on the left. Scalebar in A, 30 ﰂm. |
