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prdm13xenopus ventricular zone 

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Experiment details for prdm13

Hanotel J et al. (2014) Assay

The Prdm13 histone methyltransferase encoding gene is a Ptf1a-Rbpj downstream target that suppresses glutamatergic and promotes GABAergic neuronal fate in the dorsal neural tube.

Gene Clone Species Stages Anatomy
prdm13.L laevis NF stage 14 to NF stage 32 ventricular zone

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  Fig. S3. Mapping of Prdm13 expression domain the developing neural tube. (A-C) Double in situ hybridization of Prdm13 with Eng2, Krox-20 or Mafb at the indicated stages. Krox-20 is in magenta; Mafb, in light blue. Both probes in panel A are revealed in dark blue. Note that Prdm13 is caudal to Eng2 and Krox-20, and that its expression terminates at the domain of Mafb expression. (D-H) Double labeling of Prdm13 with N-tubulin, Dbx1 or Prdm8 at the indicated stages. In panel D and F, Prdm13 is in light blue and the other probe is in dark blue. In panel E, G and H , both Prdm13 and Prdm8 are in dark blue. Note that in the neural plate Prdm13 is detected in the region of the differentiating primary interneurons, more laterally than Prdm8 and at the lateral border of the Dbx1 domain. At neural plate stage, Prdm13 is dorsal to Dbx1.

Gene Clone Species Stages Anatomy
prdm13.L laevis NF stage 32 ventricular zone

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  Fig. 1. HMTase activity and developmental expression of Prdm13. (A) mPrdm13 is a histone methyl-transferase. Core histones were used as substrates in a HMTase assay with a immunopurified Flag-Prdm13 protein produced in 293T cells. Flag-Prdm16 was used as a positive control. Empty vector transfected cells were used to control for nonspecific background. The results represent the average of triplicate samples plus SD. The amount of immunoprecipitated Prdm16 and Prdm13 proteins used in the assay is shown on the western on the right (arrows). Note that mPrdm13 is expressed at a much lower level than mPrdm16. On the autoradiograph shown, multiple bands are seen for mPrdm16 due to the high exposure time used to detect mPrdm13. Those bands likely correspond to mPrdm16 degradation products. A non-specific IgG band is indicated by an arrowhead. (B) Temporal embryonic expression of Xenopus laevis Prdm13 as determined by RT-PCR. Histone H4 is shown as a loading control. (C–K) Whole-mount in situ analysis of Prdm13 expression during embryogenesis. Panel E, transversal section of the neural tube of a stage 22 embryo. Panels H and I, transversal sections of the neural tube of a stage 32 embryo at the levels indicated in G. In panel K, transversal section of the neural tube of an embryo that has been additionally stained for proliferation by EdU incorporation. The enlargement in the inset shows that some Prdm13 are EdU positive. Nieuwkoop Faber stages are indicated. All embryos shown are with anterior to the right. (C, D) Dorsal views. (F, G, J) Lateral views.

Gene Clone Species Stages Anatomy
prdm13.L laevis NF stage 32 to NF stage 33 and 34 ventricular zone

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  Fig. 2. Prdm13 embryonic expression profile is highly related to that of Ptf1a and overlaps with those of its downstream targets Neurog2 and Pax2 in Xenopus embryos. (A–F) Dorsal or lateral views of Prdm13 and Ptf1a in stage 18, 27 and 33 embryos showing that the two genes have a similar restricted expression in the neural tube. (G–I) Images of transversal sections of the neural tube at the level of the anterior spinal cord of a stage 32 embryo, following double fluorescent in situ hybridization for Prdm13 and Ptf1a. Note the overlap of the two stainings (arrows). (J–M) Transversal sections of the neural tube of stage 32 embryos at the level of the otic vesicles stained with the indicated probes. Note that Ptf1a appears coexpressed with Prdm13, Neurog2 and Pax2 in the dorsal neural tube. While Ptf1a is expressed in the lateral part of the ventricular zone and in the intermediate zone, Prdm13 and Neurog2 strongest expression is detected in the intermediate zone and Pax2 in the marginal zone. Nieuwkoop Faber stages are indicated.

Gene Clone Species Stages Anatomy
prdm13.L laevis NF stage 32 ventricular zone

  prdm13 (PR domain containing 13) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 32, transverse section, dorsal up.

Gene Clone Species Stages Anatomy
prdm13.L laevis NF stage 32 ventricular zone

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  prdm13 (PR domain containing 13) and dbx1 (developing brain homeobox 1) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 32, transverse section, dorsal up.

Gene Clone Species Stages Anatomy
prdm13.L laevis NF stage 32 ventricular zone

  prdm13 (PR domain containing 13) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 32, transverse section, dorsal up.

Gene Clone Species Stages Anatomy
prdm13.L laevis NF stage 32 ventricular zone

  prdm13 (PR domain containing 13) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 32, transverse section, dorsal up.