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Fig. 3. (A–I) Expression of XPtc1 during Xenopus development. Whole-mount in situ hybridization was performed on neurula and tailbud stage embryos using XPtc1 antisense RNA. Nieuwkoop-Faber stages of embryogenesis are indicated in lower right corners. (A,B) Dorsal view; anterior is left. (C) Transverse section of (B). (D) Parasagittal section of (E) at the level of the somites. One somite unit is marked by a bracket. (E) Lateral view, anterior is left. (F,G) Transverse sections. Dashed lines in (E) indicate the relative position of the section planes in (F) and (G). (H) Ventral view of (E). (I) Dorsal view of (E). Abbreviations: cg, cement gland; ev, eye vesicle; fp, floor plate; hc, hypochord; pm, paraxial mesoderm; nc, notochord; ne, neuroectoderm; nt, neural tube; ov, otic vesicle; os, optic stalk; pa, pituitary anlage; s, somite; me, mesencephalon; vz, ventricular zone. (J) Expression of XSmo at tailbud stage (st. 26). Whole-mount in situ hybridization was performed using XSmo antisense RNA from two partial cDNAs. Lateral view, anterior is left. (K) RT-PCR analysis of XSmo and XPtc1 expression in adult organs and tissues. Tissues and organs employed for RNA preparations are indicated above each column. RT-PCR was performed using the following primers: XPtc1 (F,5′-CTAAAGCGCACAGGAGCAAGC-3′ and R,5′-CAGGCT GTAGCGTGTATTGTC-3′; 303 bp; 28 cycles), XSmo (F,5′-GCCCTGGGCACCACA CATCAG-3′ and R,5′-GATTAGCACAAAGCCAAAGGC-3′; 367 bp; 28 cycles) and histone H4 (F,5′-CGGGATAACATTCAGGGTATCACT-3′ and R,5′-ATCCATGGCGGT AACTGTCTTCCT-3′; 220 bp; 22 cycles). |
