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Figure 5. Whole-mount in situ hybridization analysis of Xenopus tropicalis peroxidasin expression during development. Whole-mount in situ hybridizations showing the expression of Xtpxn at various stages. A: Side view of a zygote at 60 min postfertilization. The animal hemisphere is to the top. B: Cut face view of a zygote bisected along the animal vegetal axis. The animal hemisphere is to the top. C: Side view of two-cell stage embryo. The animal hemisphere is to the top. D: An animal view of a 32-cell stage embryo. E: Cut face view of a blastula stage 9 bisected along the animal vegetal axis. The animal hemisphere is to the top. F: Dorsal view of a neurula stage 17 embryo. Anterior is to the left. G: Lateral view of a neurula stage 17 embryo. Anterior is to the left. H: Lateral view of stage 23 embryo. Anterior is to the left. I: Lateral view of a early tailbud stage 27 embryo. J: High-power dorsal view of the hindbrain region of an early tailbud stage 27 embryo. K: Lateral view of a tailbud stage 31 embryo. Anterior is to the left. L: High-power view of the developing pronephros on the flank of a tailbud stage 31 embryo. M: The anterior region of a tailbud stage 34 embryo. N: A dorsal view of the midbrain/hindbrain region of a tailbud stage 34 embryo. O: The posterior region of a tailbud stage 34 embryo. P: High-power view of the developing pronephros on the flank of a tailbud stage 34 embryo. Q: A 10- mu m transverse section through a stage 34 embryo at the level of the pronephros. R: High-power view of 10- mu m transverse section through pronephric duct of a stage 34 embryo. bcl, blastocoele cavity; bra, branchial arches; end, endoderm; eye, eye; hb, hindbrain; mhj, midbrain/hindbrain junction; nne, non-neural ectoderm; nt, neural tube; pcd, proctodeum; pf, posterior fin; pnd, pronephric duct; pnt, pronephric tubule; tlb, tail bud. |
