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Fig. 4. Expression pattern of Ras-dva-2 in Xenopus laevis tadpoles. (A and B) In situ hybridisation of the whole tadpole at stage 46. The tadpole is shown from the dorsal (A) and right (B) sides. Ras-dva-2 expressing cells are localised to the brain, eyes and mesonephros. Additionally, weak expression can also be detected in the stomach. (C and D) The dorsal and lateral views of the dissected brain (stage 45) after the in situ hybridisation. The red asterisk indicates the rostral end of the brain. Ras-dva-2 is expressed in the diencephalon – in thalamic nucleus, preoptic nucleus and in pretectum nucleus, in the mesencephalon – within the optic tectum; and also in some cell clusters located in the hindbrain (arrowheads). The lines in D indicate the positions of transverse sections shown in F, G and H. (E) The scheme of the brain units at stage 45. Dorsal side up, the red asterisk indicates the rostral end of the brain. (F) The transverse section of the diencephalon shows Ras-dva-2 expression in the ventromedial thalamic nucleus, posteroventral division of lateral thalamic nucleus and preoptic nucleus. (G) On the transverse section of the mesencephalon Ras-dva-2 positive cells mark the optic tectum, anterodorsal and anteroventral tegmental nuclei. (H) The transverse section of the hindbrain shows Ras-dva-2 expression in nucleus descendens and nucleus motorius nervi trigemini. (I) On the transverse section of the eye Ras-dva-2 expression can be detected in some layers of the retina. (K) Nuclei staining by haematoxylin shows that the Ras-dva-2 expressing layers correspond to the inner nuclear and inner plexiform layers (INL and IPL) of the retina. Abbreviations as in Fig. 1. Additionally: Ad – anterodorsal tegmental nucleus, Av – anteroventral tegmental nucleus, Lpv – lateral thalamic nucleus, pars posteroventralis, Mn – mesonephros, Ot – optic tectum, Pon – preoptic nucleus, Ptn – pretectum nucleus, Tn – thalamic nucleus, VM – ventromedial thalamic nucleus, Vd – nucleus descendens nervi trigemini, Vm – nucleus motorius nervi trigemini. |
