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Fig. 3. BMP is required for the specification of hemogenic endothelium before stage 28. (A) Runx1 WISH of heat-shock time course. Runx1 expression is abolished in HS:Noggin siblings when BMP signaling is inhibited before stage 28 (blue box). Arrows mark runx1 expression along the DA, with green arrows indicating normal expression levels and red arrows indicating absent or decreased expression. Expression of runx1 is unaffected when the treatment is started at stage 28 or afterward. Runx1 is also expressed in lateral line nerves (*) as well as in some motor neurons (*), the staining of which in some images is overlaid with runx1 staining in the DA due to embryo positioning and focus. (B) Efnb2 expression is strongly reduced in HS:Noggin siblings when BMP signaling is blocked before stage 28. (C) Dll4 expression is relatively normal in HS:Noggin siblings. However, the DA is not properly lumenized (*) when BMP signaling is inhibited before stage 28. Embryos were visualized from the lateral view, zoomed in to the trunk region, with anterior to the left and dorsal at the top. Proportion of embryos which the image represents is shown in bottom right corner. (Scale bars: 0.5 mm.) |
