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Display additional annotations [+]
| Gene |
Clone |
Species |
Stages |
Anatomy |
|
twist2
|
|
tropicalis
|
NF stage 51
|
epidermis
,
epidermis inner layer
,
mesenchyme
,
hindlimb bud
,
limb bud
,
[+]
|
|
twist2
|
|
tropicalis
|
NF stage 52
to
NF stage 54
|
epidermis
,
epidermis outer layer
,
epidermis inner layer
,
muscle
,
mesenchyme
,
[+]
|
|
| |
Figure 2. A–D: Expression of scleraxis in the developing Xenopus limb bud. A: Stage 51. A': Higher magnification of A. Scleraxis transcriptions were observed in the subectodermal region. B:The scleraxis expression domain became restricted along the developing muscle at stage 52. C: A more restricted pattern of scleraxis expression was observed at stage 53. In the proximal subectodermal region, scleraxis transcription was down-regulated. D: Visualization of muscle and scleraxis by immunostaining and in situ hybridization. Differentiating muscles were visualized by MF20 antibody (Brown). The scleraxis expression domain was restricted to the muscle extremity (inset). Arrows indicated scleraxis expression in the putative ligament. E–H: Expression of dermo-1 in the developing Xenopus limb bud. E: In the early developing Xenopus limb bud, the expression pattern of dermo-1 was similar to that of scleraxis (compare with A). E': Higher magnification of E shows dermo-1 transcription in the subectodermal region that was gradually down-regulated toward the medial region (arrow). F,G: In stages 52 and 53, subectodermal cells dominantly expressed dermo-1. The dermo-1 expression pattern in the proximal subectodermal region was more restricted (G' is compared with E', arrow). H: Stage 54. dermo-1 transcription was observed not only in the subectodermal region but also in some other regions. Scale bar = 500 μm. |
