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Fig. 5. In situ analysis of Xsyn-2 RNA expression during development. (A) Lateral view of blastula (stage 7); animal pole is to the top, Xsyn-2 is ex- pressed in the animal hemisphere, the future ectoderm. (B) Animal-vegetal section of blastula stage embryo, confirming localization of Xsyn-2 mRNA to the animal hemisphere. Animal is to the top. (C) Animal-vegetal section of a midgastrula embryo (stage 11.5) showing Xsyn-2 expression in the sensorial layer of the ectoderm. Arrowheads denote the boundaries of Xsyn-2 expression at the blastopore lip. Animal pole is to the top. (D) Lateral view of early neurula (stage 13); anterior is to the tight, dorsal is to the top. This embryo is not cleared, so only surface staining is visible. Xsyn-2 ex- pression is now restricted to the neural plate, and is not found in lateral or ventral ectoderm. nb, neural plate boundary. (E) Dorsal view of midneurula (stage 15); anterior is to the right. Xsyn-2 expression is restricted to the neural midline (nm) and to the anterior neural folds. (F) Lateral view of early tailbud stage embryo (stage 28): anterior is to the right, dorsal is to the top. Xsyn-2 is expressed at relatively high levels in the midbrain-hindbrain and midbrain-forebrain boundaries (arrowheads), and in the otic vesicles (ot). Lower levels of expression are seen in the floorplate of the neural tube, eye, tailbud. somites, pharyngeal mesoderm, pronephros and pronephric duct. (G) Lateral view of mid-taiibud stage embryo (stage 33); anterior is to the right, dorsal is to the top. This embryo was overstained to show Xsyn-2 expression in the heart (h). The cardiac tube is justeginning to loop at this stage. (H) Stage 33 sense control. This embryo was overstained as in (Cl). Faint background is present in the brain and pharyngeal mesoderm. Sense controls for all other stages had no background. Bars, 0.5 mm. Bar in (A) applies to (B-E,G.H). |
