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Fig. 2. Expression patterns of xSH3BP5L. (A) Temporal expression profile of xSH3BP5L analyzed by RT-PCR. Transcripts of xSH3BP5L were detected from unfertilized egg to tadpole. RT-PCR using an ornithine decarboxylase (ODC)-specific primer was carried out in parallel to control the amount of input RNA (ODC (RT+)]. RT-PCR without reverse transcriptase showed no contamination of genomic DNA (ODC (RT−)]. (B) Tissue expression pattern of xSH3BP5L in adult frog analyzed by RT-PCR. (C–G) Whole-mount in situ hybridization reveals spatial distribution patterns of xSH3BP5L mRNA. Bars, 250 μm. (C,D) xSH3BP5L expression is detectable in animal pole cells of blastula embryos. (E) xSH3BP5L mRNA is expressed in the ventral–lateral mesoderm during early gastrulation. (F) Expression of xSH3BP5L in the head. (G) Lateral view of a stage-29 embryo, showing that xSH3BP5L mRNA is detected predominantly in eye, but faintly in bronchial arch, otic vesicle, and pronephros (arrowheads in G). (H) Embryo hybridized with Xmyod antisense probe showing muscle signals (black arrowheads). (I) Control hybridization at stage-30 with sense xSH3BP5L probe. Ba, bronchial arches; ev, eye vesicle; m, muscle; ov, otic vesicle; pr, pronephros. |
