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Fig. 3. Expression pattern of Xshisa-2 during tailbud stages. From early tailbud stage onward, a dynamic pattern is observed in the forming
somites. (A-B) At stage 18, Xshisa-2 becomes progressively reduced in the anterior paraxial mesoderm. (B’) Transverse section showing Xshisa-2
expression in the entire somite region. (C) Parasagittal section of a stage 18 embryo. (D) Double whole mount in situ hybridization with Xshisa-2 DIGlabeled
antisense RNA probe and XmyoD fluo-labelled antisense RNA probe. The expression domain of XmyoD extends more posteriorly than the
Xshisa-2 expression domain. (E-H,J-K) Expression of Xshisa-2 is stronger in the presomitic mesoderm and decreases as somites form. Transverse
sections through the trunk region of stage 27 (G’’) and 30 (J’) embryos show Xshisa-2 expression in the entire somite. (H,H’) Double whole mount in
situ hybridization with Xshisa-2 and PAPC shows that Xshisa-2 is not expressed in the unsegmented region of the presomitic mesoderm. (I) Whole mount
in situ hybridization with Xshisa shows expression restricted to the head region, a distinct expression pattern than the one observed for Xshisa-2. (K’)
A complex expression pattern is also observed in the head, including the lens (ln) and branchial arches (ba). (A,C,E-G,H-J,K,K’) Lateral view. (B,D,G’). Dorsal
view. All embryos are oriented with anterior to the right. |
