| |
FIG. 5. Comparison in stage 40 Xenopus retina of X-Notch-1 expression with that of genes involved in early eye development. (A) Comparison of X-Notch-1 and XSix3 expression patterns. Double in situ hybridizations were performed as in Fig. 1 with XSix3 in deep purple and X-Notch-1 in red. (A) XSix3 is expressed in the CMZ, from the most peripheral cells of the retina, and in the central retina, in the ganglion cell layer and the inner nuclear layer. (B) X-Notch-1 labeling is detected in the CMZ except in cells in the most peripheral region. C and D show a higher magnification of the CMZ from the sections depicted in A and B, respectively. (E) Double labeling demonstrates that XSix3 expression starts more in the periphery than X-Notch-1 expression. Scale bar in A and B, 100 ﰂm; scale bar in C, D, and E, 30 ﰂm. (F) Comparison of X-Notch-1 and Xrx1 expression patterns. Double in situ hybridizations were performed as in Fig. 1 with Xrx1 in deep purple and X-Notch-1 in red. (F) Xrx1 is expressed in the CMZ, from the most peripheral cells of the retina, and in the central retina, in a subset of cells in the outer part of the inner nuclear layer and in the outer nuclear layer. (G) X-Notch-1 labeling is detected in the CMZ except in cells in the most peripheral region. H and I show a higher magnification of the CMZ from the sections depicted in F and G, respectively. (J) Double labeling demonstrates that Xrx1 expression starts more in the periphery than X-Notch-1 expression. Scale bar in F and G, 100 ﰂm; scale bar in H, I, and J, 30 ﰂm. (K) Comparison of X-Notch-1 and Pax6 expression patterns. Double in situ hybridizations were performed as in Fig. 1 with Pax6 in deep purple and X-Notch-1 in red. (K) Pax6 is strongly expressed in the central retina, in the ganglion cell layer and in the inner part of the inner nuclear layer, and faint staining is also detected throughout the CMZ. (L) X-Notch-1 labeling is detected in the CMZ except in cells in the most peripheral region. M and N show a higher magnification of the CMZ from the sections depicted in K and L, respectively. (O) Double labeling demonstrates that the strong expression of Pax6 and X-Notch-1 expression does not overlap but that the faint expression of Pax6 in the CMZ starts more peripherally than X-Notch-1 expression. Scale bar in K and L, 100 um; scale bar in M, N, and O, 30 um. |
