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Fig. 1. Spatial and temporal expression patterns of xGlyT1, xGlyT2, and xVIAAT in developing embryos using whole mount in situ hybridization. Embryos are viewed laterally, with anterior to the left and dorsal to the top. (A–C) Stages 21–22 (late neurula). (D–F) Stages 24–25 (early tailbud). (G–I) Stages 27–29 (late tailbud). (J–L) Stages 31–32 (early hatching). (M–O) Stages 33–34 (late hatching). (P–R) Stages 35–37 (swimming tadpole). (A, D, G, J, M, P) xGlyT1 expression; (B, E, H, K, N, Q) xGlyT2 expression; (C, F, I, L, O, R) xVIAAT expression. Hollow arrowheads denote telencephalic xVIAAT expression; solid arrowheads denote diencephalic xVIAAT expression. Abbreviations: fb, forebrain; hb, hindbrain; mb, midbrain; sc, spinal cord; so, somite. Scale bars represent 1.0 mm. |
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Fig. 4. Histological analysis of xVIAAT mRNA expression in stages 35–37 (swimming tadpole) X. laevis using whole mount in situ hybridization. (A–H) Transverse 10-μm sections along the anterior–posterior axis, with dorsal to the top. (E′–H′) Magnified images of the neural tube from the above mentioned sections. Abbreviations: cg, cement gland; e, eye; fb, forebrain; fg, foregut; hb, hindbrain; mb, midbrain; no, notochord; ot, otic vesicle; sc, spinal cord; so, somite. Scale bars represent 1.0 mm for A–H and 0.25 mm for E′–H′. |
