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Figure 9. Immunostaining of activated Smads across the dorsoventral axis. Immunostaining of activated Smads across the dorsoventral axis. Stage 10+ embryos were fixed and bisected for immunohistochemistry. Dorsoventral orientation was determined by the dorsal blastopore lip, which is indicated with an
arrow in (A-D). Embryo diameter is
approximately 1 mm. Immunostaining
with anti-phosphoSmad1 antibody was carried out with uninjected embryos (A) or embryos injected marginally with noggin (100 pg/embryo) (B) or BMP4 (500 pg/embryo) (C). AntiphosphoSmad2 immunostaining was performed with uninjected embryos (D) or embryos injected marginally with delta1XAR1 (1 ng/embryo) (E) or activin (50 pg/embryo) (F). |
