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Fig. 2. Expression patterns of neural crest markers and neural crest inducers in Tbx6 knockdown embryos as revealed by whole-mount in situ hybridization. MO-injected sides (*) are shown by Magenta-GAL staining. Images of MO-injected sides are flipped horizontally to facilitate comparison. (A) Snail2 and Sox9 expressions at stage 16. Dorsal view, anterior to top. (B) Fgf8 and Wnt8 expressions at stage 14. Dorsal view, anterior to top. (C) Snail2, Sox9, and PCNS expressions in the head of stages 19, 25, and 27 embryos. Lateral view, anterior to the left. (D) Tbx6 expression at stage 47 (arrowhead). Lateral view, anterior to the left. (E) Sox9 expression in the head of stage 27 embryos injected into one cell at the 8-cell stage with 6 ng of Tbx6 exint-MO. |
