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Gene/CloneSpeciesStageAnatomy ItemExperimenter
spty2d1xenopus dorsal 

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Experiment details for spty2d1

Shibata M et al. (2001) Assay

Systematic screening and expression analysis of the head organizer genes in Xenopus embryos.

Gene Clone Species Stages Anatomy
spty2d1.S p16h6 laevis NF stage 13 dorsal

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  FIG. 3. Isolated cDNA clones expressed in the AEM region and notochord. (A) Clone names and similar genes or proteins in the public databases are indicated on the left side of each panel. Results of whole-mount in situ hybridization for early neurula (stage 13) are shown in dorsal (left) and lateral (right) view. (B) Northern blot analysis. Clone names and their closest genes or proteins in the public databases are indicated on the left side of each panel and the deduced mRNA size (kb) of each clone on the right (see Table 2 for P11F3). The developmental stages are indicated on the top of each column by numerical figures according to Nieuwkoop and Faber (1967). 18S rRNA stained with ethidium bromide is for loading control. Ten micrograms of total RNA from stage-9, -10, -12.5, and -15 embryos were electrophoresed in each lane, blotted, and hybridized with probes derived from each clone except for crescent. Crescent probe was hybridized to the same blot as previously used (Hikasa and Taira, 2001) with a wider range of stages as indicated.

Gene Clone Species Stages Anatomy
spty2d1.S p16h6 laevis NF stage 13 dorsal

  spty2d1 (SPT2 chromatin protein domain containing 1) gene expression in Xenopus laevis embryos, NF stage 13, as assayed by in situ hybridization. Left image: dorsal view, anterior left. Right image: lateral view of bisected embryo: anterior left, dorsal up.