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sst.1xenopus mesoderm [+] 

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Experiment details for sst.1

Oropez D and Horb M (2012) Assay

Transient expression of Ngn3 in Xenopus endoderm promotes early and ectopic development of pancreatic beta and delta cells.

Gene Clone Species Stages Anatomy
sst.1.S laevis NF stage 32 mesoderm

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  Figure 5. Ngn3-GR promotes beta cell differentiation. (A) RT-PCR for insulin of whole embryos at Stages 24 and 26. (b,c) Increased pax4 expression detected in Dex treated embryos at Stage 32 (n = 15/17). (d,e) Increase in the mature beta cell marker, sur1, at Stage 32 (n = 19/31) in Dex treated embryos. In panel (d), arrow indicates nonendodermal expression of sur1. In panel (e), bracket denotes ectopic expression in endoderm. (f,g) Increase in somatostatin at Stage 32 (n = 12/27) in Dex treated embryos. In panel (F), arrow indicates expression of som outside the endoderm. In panel G, bracket denotes ectopic expression in endoderm. (h) RT-PCR at Stage 26 of whole endoderm (WE) and whole endoderm plus mesoderm explants (WEM). Explants were dissected at Stage 15 following 4 h of Ngn3-GR activation, cultured until Stage 26 and collected for RT-PCR. Twist and FoxF1 are mesoderm markers, which are detected only in the WEM explants.