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Figure 1. Xenopus STIM1 is expressed in developing neural tissues and neuronal growth cones. (A) Sample images of whole-mount (top) and cross-section (bottom) in situ hybridization analysis of the mRNA expression of XSTIM1 in developing Xenopus embryos. Left, antisense; right, sense probe. Dotted lines delineate the boundaries of neural tube and notochord. (B) RT-PCR detection of XSTIM1 mRNA from RNA samples extracted from state 25-26 Xenopus neural tube and notochord tissues. –RT lane is the negative control of the RT-PCR on neural tube tissue RNA in the absence of a reverese transcriptase. (C) Representative immunofluorescence images of cultured Xenopus spinal neurons labeled for STIM1 (red) and F-actin (phalloidin: green). Scale bar: 20 μm. (D) Representative immunofluorescence images of growth cones labeled for STIM1 (red) and F-actin (green). Negative control processed without STIM1 antibody (without STIM1, bottom) shows absence of immunolabeling. Scale bar: 10 μm. |
